rabbit anti mouse cd38  (Bioss)

Journal: bioRxiv

Article Title: Effect of Thrombospondin-4 on Pro-inflammatory Phenotype Differentiation and Apoptosis in Macrophages

doi: 10.1101/633537

Figure Lengend Snippet: BMDM were treated with LPS (0.5 μg/mL) and IFNγ (1000 IU/mL) for 6 hours, and Nos2 and CD38 mRNA expression was measured by QRT-PCR; n = 3; *p <0.05.

Article Snippet: Sections of mice peritoneum were stained with primary rat anti-mouse CD68 antibody (MCA1957B, Bio-Rad), rabbit anti-mouse CD38 antibody (bs-0979R, Bioss Antibodies), Rabbit anti-mouse Egr2 (ab90518, Abcam) antibody using Vecta Stain ABC Kit.

Techniques: Expressing, Quantitative RT-PCR

Journal: bioRxiv

Article Title: Effect of Thrombospondin-4 on Pro-inflammatory Phenotype Differentiation and Apoptosis in Macrophages

doi: 10.1101/633537

Figure Lengend Snippet: A: Expression of CD68, CD38, and Egr2 was measured in peritoneal tissues of WT mice with LPS-induced peritonitis; n=3; *p<0.05. B: Expression of CD68 and CD38 was measured in peritoneal tissues of Thbs4 −/− mice with LPS-induced peritonitis; n=3; *p<0.05. C: Expression of CD68 and CD38 was measured in peritoneal tissues of P387-TSP4-KI mice with LPS-induced peritonitis; n=3; *p<0.05. D: Migration of RAW264.7 was measured in Boyden chambers (uncoated, coated with A387 or P387 rTSP-4, or BSA); n=3; *p<0.05 compared to plastic and BSA; #p<0.05 compared to WT rTSP-4. E: A387 and P387 rTSP-4 was used in adhesion competition assay: plastic was coated with P387 rTSP-4, and RAW264.7 were added in increasing concentrations; n=3; *p<0.05.

Article Snippet: Sections of mice peritoneum were stained with primary rat anti-mouse CD68 antibody (MCA1957B, Bio-Rad), rabbit anti-mouse CD38 antibody (bs-0979R, Bioss Antibodies), Rabbit anti-mouse Egr2 (ab90518, Abcam) antibody using Vecta Stain ABC Kit.

Techniques: Expressing, Migration, Competitive Binding Assay